Effect of low-dose Gamma Knife radiation on amino acid transmitters in the hippocampus of epileptic ratJunchao Liang1, Qingsheng Xu2, Ke Ye2, Renya Zhan21 2Junchao Liang , Dengfeng WU , Weimin Wang , Youliang WU Keywords: epilepsy, radiobiology, gamma knife, glutamate, radiosurgeryInteractive Manuscript
Ask Questions of this Manuscript:
What is the background behind your study?
The radiobiological effect of radiosurgery for seizure control remains to be elucidated.
What is the purpose of your study?
Our purpose was to explore the mechanism of Gamma Knife radiosurgery for epilepsy by
studying the effect of low-dose Gamma Knife radiosurgery on glutamate
(Glu) and ?-aminobutyric acid (GABA) in the hippocampus of epileptic
rats.
Describe your patient group.
This question was not answered by the author
Describe what you did.
Fifty Li-pilocarpine-induced epileptic rats were randomly divided equally into radiation group and non-radiation group. Additional twenty-five normal rats were treated with normal sodium as the control group. The radiation group rats were treated by Gamma Knife radiation at a marginal dose of 20 Gy and no radiation was performed in non-radiation and control groups. Glu and GABA contents in the hippocampus of epileptic rats were detected by high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS-MS) in all groups.
Describe your main findings.
After two weeks of radiation, the contents of Glu in the hippocampus of the rats in non-radiation and radiation groups were significantly higher than control group, and that of radiation group was significantly lower than non-irradiation group (P<0.05). The contents of GABA in the hippocampus of the rats in on-irradiated and irradiated group were significantly lower than control group, and that of radiation group was significantly higher than non-irradiated group (P<0.05).
Describe the main limitation of this study.
The findings of this study may be specific to this model.
Describe your main conclusion.
Low-dose Gamma Knife irradiation can counteract epilepsy through the regulation of the balance of Glu and GABA in the hippocampus.
Describe the importance of your findings and how they can be used by others.
There may be other mechanisms of action not studied in this experiment.
The radiobiological effect of radiosurgery for seizure control remains to be elucidated.
Our purpose was to explore the mechanism of Gamma Knife radiosurgery for epilepsy by
studying the effect of low-dose Gamma Knife radiosurgery on glutamate
(Glu) and ?-aminobutyric acid (GABA) in the hippocampus of epileptic
rats.
Fifty Li-pilocarpine-induced epileptic rats were randomly divided equally into radiation group and non-radiation group. Additional twenty-five normal rats were treated with normal sodium as the control group. The radiation group rats were treated by Gamma Knife radiation at a marginal dose of 20 Gy and no radiation was performed in non-radiation and control groups. Glu and GABA contents in the hippocampus of epileptic rats were detected by high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS-MS) in all groups.
After two weeks of radiation, the contents of Glu in the hippocampus of the rats in non-radiation and radiation groups were significantly higher than control group, and that of radiation group was significantly lower than non-irradiation group (P<0.05). The contents of GABA in the hippocampus of the rats in on-irradiated and irradiated group were significantly lower than control group, and that of radiation group was significantly higher than non-irradiated group (P<0.05).
The findings of this study may be specific to this model.
Low-dose Gamma Knife irradiation can counteract epilepsy through the regulation of the balance of Glu and GABA in the hippocampus.
There may be other mechanisms of action not studied in this experiment.
Project Roles:
J. Liang (), Q. Xu (), K. Ye (), R. Zhan ()
