Early Effects Of Gamma Knife On Synaptophysin And Mossy Fiber Sprouting In Hippocampus Of Rat Temporal Lobe Epilepsy ModelKeywords: gamma knife, animal model, epilepsy, hippocampectomy, radiobiologyInteractive Manuscript
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What is the background behind your study?
What is the purpose of your study?
To investigate early effects of gamma knife on synaptophysin(SYN) expression and mossy fiber sprouting(MFS) in pilocarpine-induced model of temporal lobe epilepsy and explore the mechanisms of Gamma Knife radiosurgery for temporal lobe epilepsy, and so as to provide a theoretical basis for clinical practice.
Describe your patient group.
All Wistar rats were divided randomly into three groups: blank control group, treatment group and non-treatment group.
Describe what you did.
Developed temporal lobe epilepsy model induced by lithium-pilocarpine. Treatment group rats were treated by Leksell Gamma Knife on 2w after status epilepticus. Irradiation target was the left hippocampus; the central dose was 40Gy at the isodose line 70%.Immunohistochemistry and Timm staining were used to observe the expression of SYN and MFS in the inner molecular layer (IML).
Describe your main findings.
As compared with the non-treatment group ,treated rats showed reductions in the number of spontaneous seizures in the latter of the study (Weeks 6-8). The expression of SYN in IML was increased compared with blank control group. As compared with the non-treatment group, treatment group SYN particles had different degrees of reduction in the left dentate gyrus IML. No significant difference between the two groups on 2h, (P>0.05); 2w-8w later difference between the two groups was statistically significant (P<0.05);treatment group the Timm staining score in the left dentate gyrus IML have different degrees of reduction, no significant difference between the two groups from 2h to 4w(P>0.05);8w later, it became significantly different(P<0.05).
Describe the main limitation of this study.
This is a retrospective study.
Describe your main conclusion.
Gamma Knife irradiation can inhibit the expression of SYN and reduce the MFS in IML in early stages.
Describe the importance of your findings and how they can be used by others.
Such a change may be one of the important mechanisms of Gamma Knife treatment of temporal lobe epilepsy.
To investigate early effects of gamma knife on synaptophysin(SYN) expression and mossy fiber sprouting(MFS) in pilocarpine-induced model of temporal lobe epilepsy and explore the mechanisms of Gamma Knife radiosurgery for temporal lobe epilepsy, and so as to provide a theoretical basis for clinical practice.
All Wistar rats were divided randomly into three groups: blank control group, treatment group and non-treatment group.
Developed temporal lobe epilepsy model induced by lithium-pilocarpine. Treatment group rats were treated by Leksell Gamma Knife on 2w after status epilepticus. Irradiation target was the left hippocampus; the central dose was 40Gy at the isodose line 70%.Immunohistochemistry and Timm staining were used to observe the expression of SYN and MFS in the inner molecular layer (IML).
As compared with the non-treatment group ,treated rats showed reductions in the number of spontaneous seizures in the latter of the study (Weeks 6-8). The expression of SYN in IML was increased compared with blank control group. As compared with the non-treatment group, treatment group SYN particles had different degrees of reduction in the left dentate gyrus IML. No significant difference between the two groups on 2h, (P>0.05); 2w-8w later difference between the two groups was statistically significant (P<0.05);treatment group the Timm staining score in the left dentate gyrus IML have different degrees of reduction, no significant difference between the two groups from 2h to 4w(P>0.05);8w later, it became significantly different(P<0.05).
This is a retrospective study.
Gamma Knife irradiation can inhibit the expression of SYN and reduce the MFS in IML in early stages.
Such a change may be one of the important mechanisms of Gamma Knife treatment of temporal lobe epilepsy.
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